Biochemistry
Lecture 18 (Handout): DNA Replication & Repair Enzymes
Worksheet Answers for some of the enzymes and other proteins that
act on DNA.
Enzyme
or protein |
|
DNA Pol I
(polymerase) |
Nick
translation |
Fill-in gap |
None |
None |
| Pol I 3'->5' exo |
3' mismatch
hydrolyzed |
3' mismatch
hydrolyzed |
None |
None |
| Pol I 5'->3' exo |
None |
None |
Removed by
nick translation |
Removed by
nick translation |
| DNA ligase |
Seals nick |
None |
None |
None |
| DNA Pol III |
None |
Fill-in gap |
None |
None |
| SSB |
None |
Binds tightly |
None |
None |
| primase |
None |
Makes an
RNA primer |
None |
None |
| helicase |
None |
Loads at 5' end |
None |
None |
Eukaryotic
Pol a |
3' mismatch
hydrolyzed |
Synthesis on
lagging strand |
None |
None |
Eukaryotic
Pol d |
3' mismatch
hydrolyzed |
Synthesis on
leading strand |
None |
None |
| photolyase |
None |
None |
Removes dimer
leaving a gap |
None |
UvrABC
endonuclease |
None |
None |
Removes mismatch
leaving a gap |
None |
| |
|
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